quantigene 1.0 branched dna (bdna) assay kit Search Results


1.6 kb segment of the human bdnf cdna (bp 10– 1610 x34333)  (Regeneron inc)
90
Regeneron inc 1.6 kb segment of the human bdnf cdna (bp 10– 1610 x34333)
1.6 Kb Segment Of The Human Bdnf Cdna (Bp 10– 1610 X34333), supplied by Regeneron inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1.6 kb segment of the human bdnf cdna (bp 10– 1610 x34333) - by Bioz Stars, 2026-11
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hbv dna test versant 3.0  (Bayer HealthCare Pharmaceuticals Inc)
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Bayer HealthCare Pharmaceuticals Inc hbv dna test versant 3.0
Hbv Dna Test Versant 3.0, supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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branched dna (bdna) signal amplification assay  (Siemens AG)
90
Siemens AG branched dna (bdna) signal amplification assay
SFV status in monkeys by PCR analysis. Peripheral blood mononuclear cell (PBMC) <t>DNA</t> was isolated from 24 monkeys at 5 months prior to initiation of the study and analyzed by a nested PCR assay using SFV set B primers resulting in a 390 bp fragment. PCR <t>amplification</t> of a ~300 bp Mamu-DRB gene fragment was used as a control to determine the presence of intact DNA in the test samples.
Branched Dna (Bdna) Signal Amplification Assay, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantigene+1%2E0+branched+dna+(bdna)+assay+kit/quantitative+branched+dna+signal+amplification+assay/pmc03717714-143-15-23
Average 90 stars, based on 1 article reviews
branched dna (bdna) signal amplification assay - by Bioz Stars, 2026-11
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versant hbv dna 3.0  (Bayer HealthCare Pharmaceuticals Inc)
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Bayer HealthCare Pharmaceuticals Inc versant hbv dna 3.0
SFV status in monkeys by PCR analysis. Peripheral blood mononuclear cell (PBMC) <t>DNA</t> was isolated from 24 monkeys at 5 months prior to initiation of the study and analyzed by a nested PCR assay using SFV set B primers resulting in a 390 bp fragment. PCR <t>amplification</t> of a ~300 bp Mamu-DRB gene fragment was used as a control to determine the presence of intact DNA in the test samples.
Versant Hbv Dna 3.0, supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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gene exp cx3cl1 rn00593186 m1  (Thermo Fisher)
93
Thermo Fisher gene exp cx3cl1 rn00593186 m1
SFV status in monkeys by PCR analysis. Peripheral blood mononuclear cell (PBMC) <t>DNA</t> was isolated from 24 monkeys at 5 months prior to initiation of the study and analyzed by a nested PCR assay using SFV set B primers resulting in a 390 bp fragment. PCR <t>amplification</t> of a ~300 bp Mamu-DRB gene fragment was used as a control to determine the presence of intact DNA in the test samples.
Gene Exp Cx3cl1 Rn00593186 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantigene+1%2E0+branched+dna+(bdna)+assay+kit/Gene+Exp%2E+Cx3cl1%2C+Rn00593186_m1/ppr0341534-407-20-7
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abs against bdnf  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology abs against bdnf
Figure 6. <t>Excess</t> <t>maternal</t> fructose intake reduces hippocampal <t>BDNF</t> and TrkB protein expression at PD21. A) The effect of excess maternal fructose intake on hippocampal protein expression at weaning was assessed via Western blotting. Maternal fructose intake was associated with significant decreases in the relative protein expression of TrkB, pTrkB, Akt, pAkt, and BDNF at PD21 (n = 4–5/group). B) At PD 60, no significant differences in protein levels were observed between the C group and F group (n = 5/group). C, D) Ratios of pAkt to Akt and pTrkB to TrkB in the F group are shown relative to those of the C group at PD21. The ratio of pAkt to Akt was decreased in offspring of the F group (n = 4–5/group). E, F) There were equivalent levels of pAkt/Akt and pTrkB/TrkB at PD60 in both groups (n = 5/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.
Abs Against Bdnf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abs against bdnf - by Bioz Stars, 2026-11
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quantiplex branched dna assay  (Bayer HealthCare Pharmaceuticals Inc)
90
Bayer HealthCare Pharmaceuticals Inc quantiplex branched dna assay
Figure 6. <t>Excess</t> <t>maternal</t> fructose intake reduces hippocampal <t>BDNF</t> and TrkB protein expression at PD21. A) The effect of excess maternal fructose intake on hippocampal protein expression at weaning was assessed via Western blotting. Maternal fructose intake was associated with significant decreases in the relative protein expression of TrkB, pTrkB, Akt, pAkt, and BDNF at PD21 (n = 4–5/group). B) At PD 60, no significant differences in protein levels were observed between the C group and F group (n = 5/group). C, D) Ratios of pAkt to Akt and pTrkB to TrkB in the F group are shown relative to those of the C group at PD21. The ratio of pAkt to Akt was decreased in offspring of the F group (n = 4–5/group). E, F) There were equivalent levels of pAkt/Akt and pTrkB/TrkB at PD60 in both groups (n = 5/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.
Quantiplex Branched Dna Assay, supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantigene+1%2E0+branched+dna+(bdna)+assay+kit/quantiplex+branched+dna+assay/pmc03529240-31-8-29
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versant 3.0 branched dna assay  (Bayer HealthCare Pharmaceuticals Inc)
90
Bayer HealthCare Pharmaceuticals Inc versant 3.0 branched dna assay
Figure 6. <t>Excess</t> <t>maternal</t> fructose intake reduces hippocampal <t>BDNF</t> and TrkB protein expression at PD21. A) The effect of excess maternal fructose intake on hippocampal protein expression at weaning was assessed via Western blotting. Maternal fructose intake was associated with significant decreases in the relative protein expression of TrkB, pTrkB, Akt, pAkt, and BDNF at PD21 (n = 4–5/group). B) At PD 60, no significant differences in protein levels were observed between the C group and F group (n = 5/group). C, D) Ratios of pAkt to Akt and pTrkB to TrkB in the F group are shown relative to those of the C group at PD21. The ratio of pAkt to Akt was decreased in offspring of the F group (n = 4–5/group). E, F) There were equivalent levels of pAkt/Akt and pTrkB/TrkB at PD60 in both groups (n = 5/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.
Versant 3.0 Branched Dna Assay, supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantigene+1%2E0+branched+dna+(bdna)+assay+kit/b+dna+assay+versant+3+0/pm17608856-47-25-30
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mouse bdnf (np_001041604.1) versaclone cdna  (R&D Systems)
N/A
Mouse BDNF (NP_001041604.1) VersaClone cDNA
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Image Search Results


SFV status in monkeys by PCR analysis. Peripheral blood mononuclear cell (PBMC) DNA was isolated from 24 monkeys at 5 months prior to initiation of the study and analyzed by a nested PCR assay using SFV set B primers resulting in a 390 bp fragment. PCR amplification of a ~300 bp Mamu-DRB gene fragment was used as a control to determine the presence of intact DNA in the test samples.

Journal: Viruses

Article Title: Influence of Naturally Occurring Simian Foamy Viruses (SFVs) on SIV Disease Progression in the Rhesus Macaque ( Macaca mulatta ) Model

doi: 10.3390/v5061414

Figure Lengend Snippet: SFV status in monkeys by PCR analysis. Peripheral blood mononuclear cell (PBMC) DNA was isolated from 24 monkeys at 5 months prior to initiation of the study and analyzed by a nested PCR assay using SFV set B primers resulting in a 390 bp fragment. PCR amplification of a ~300 bp Mamu-DRB gene fragment was used as a control to determine the presence of intact DNA in the test samples.

Article Snippet: Additionally, plasma SIV RNA was determined as 10 8.9 copies per mL by a branched DNA (bDNA) signal amplification assay [ , ] (Siemens).

Techniques: Isolation, Nested PCR, Amplification, Control

SIV viral load analysis. Plasma viral load was determined at the indicated times by bDNA from weeks 2 through 34. Mean ± SEM of the results from SFV+/SIV+ and SFV−/SIV+ monkey groups was plotted 2–34 weeks. Box and whiskers analysis of the plasma viral load from 6–28 weeks and 20–28 weeks for the SFV positive and SFV negative monkey groups is shown; p values and number of animals in each group are indicated.

Journal: Viruses

Article Title: Influence of Naturally Occurring Simian Foamy Viruses (SFVs) on SIV Disease Progression in the Rhesus Macaque ( Macaca mulatta ) Model

doi: 10.3390/v5061414

Figure Lengend Snippet: SIV viral load analysis. Plasma viral load was determined at the indicated times by bDNA from weeks 2 through 34. Mean ± SEM of the results from SFV+/SIV+ and SFV−/SIV+ monkey groups was plotted 2–34 weeks. Box and whiskers analysis of the plasma viral load from 6–28 weeks and 20–28 weeks for the SFV positive and SFV negative monkey groups is shown; p values and number of animals in each group are indicated.

Article Snippet: Additionally, plasma SIV RNA was determined as 10 8.9 copies per mL by a branched DNA (bDNA) signal amplification assay [ , ] (Siemens).

Techniques: Clinical Proteomics

Figure 6. Excess maternal fructose intake reduces hippocampal BDNF and TrkB protein expression at PD21. A) The effect of excess maternal fructose intake on hippocampal protein expression at weaning was assessed via Western blotting. Maternal fructose intake was associated with significant decreases in the relative protein expression of TrkB, pTrkB, Akt, pAkt, and BDNF at PD21 (n = 4–5/group). B) At PD 60, no significant differences in protein levels were observed between the C group and F group (n = 5/group). C, D) Ratios of pAkt to Akt and pTrkB to TrkB in the F group are shown relative to those of the C group at PD21. The ratio of pAkt to Akt was decreased in offspring of the F group (n = 4–5/group). E, F) There were equivalent levels of pAkt/Akt and pTrkB/TrkB at PD60 in both groups (n = 5/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.

Journal: The FASEB Journal

Article Title: Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter

doi: 10.1096/fj.201700783rr

Figure Lengend Snippet: Figure 6. Excess maternal fructose intake reduces hippocampal BDNF and TrkB protein expression at PD21. A) The effect of excess maternal fructose intake on hippocampal protein expression at weaning was assessed via Western blotting. Maternal fructose intake was associated with significant decreases in the relative protein expression of TrkB, pTrkB, Akt, pAkt, and BDNF at PD21 (n = 4–5/group). B) At PD 60, no significant differences in protein levels were observed between the C group and F group (n = 5/group). C, D) Ratios of pAkt to Akt and pTrkB to TrkB in the F group are shown relative to those of the C group at PD21. The ratio of pAkt to Akt was decreased in offspring of the F group (n = 4–5/group). E, F) There were equivalent levels of pAkt/Akt and pTrkB/TrkB at PD60 in both groups (n = 5/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.

Article Snippet: Membrane was incubated overnight at 4°C with primary Abs against BDNF (1:100; Santa Cruz MATERNAL FRUCTOSE AND OFFSPRING BRAIN FUNCTION 2551 nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.201700783R R by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [23/10/2024].

Techniques: Expressing, Western Blot

Figure 7. Excess maternal fructose intake induces hypermethylation of the BDNF promoter region at PD21 and PD60. A) The analyzed sequence of the BDNF promoter region is shown schematically. The bisulfite-pyrosequencing method revealed that excess maternal fructose intake induced hypermethylation of the BDNF promoter region at the CREB binding site (n = 6–11/ group). B) DNA methylation upstream from the transcription start site of the TrkB gene was not altered at PD21 or PD60 (n = 6–11/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05.

Journal: The FASEB Journal

Article Title: Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter

doi: 10.1096/fj.201700783rr

Figure Lengend Snippet: Figure 7. Excess maternal fructose intake induces hypermethylation of the BDNF promoter region at PD21 and PD60. A) The analyzed sequence of the BDNF promoter region is shown schematically. The bisulfite-pyrosequencing method revealed that excess maternal fructose intake induced hypermethylation of the BDNF promoter region at the CREB binding site (n = 6–11/ group). B) DNA methylation upstream from the transcription start site of the TrkB gene was not altered at PD21 or PD60 (n = 6–11/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05.

Article Snippet: Membrane was incubated overnight at 4°C with primary Abs against BDNF (1:100; Santa Cruz MATERNAL FRUCTOSE AND OFFSPRING BRAIN FUNCTION 2551 nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.201700783R R by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [23/10/2024].

Techniques: Sequencing, Binding Assay, DNA Methylation Assay

Figure 9. Maternal fructose intake has no effect on gene/protein expression or methylation at PD160. A) Relative gene expression of BDNF was equivalent in both groups at PD160 (n = 8/group). B) No significant differences in BDNF protein expression were observed at PD160 (n = 5/group). C) There were no significant alterations in DNA methylation of the BDNF promoter at PD160 (n = 7/group). C, C group; F, F group. Values are presented as means 6 SD.

Journal: The FASEB Journal

Article Title: Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter

doi: 10.1096/fj.201700783rr

Figure Lengend Snippet: Figure 9. Maternal fructose intake has no effect on gene/protein expression or methylation at PD160. A) Relative gene expression of BDNF was equivalent in both groups at PD160 (n = 8/group). B) No significant differences in BDNF protein expression were observed at PD160 (n = 5/group). C) There were no significant alterations in DNA methylation of the BDNF promoter at PD160 (n = 7/group). C, C group; F, F group. Values are presented as means 6 SD.

Article Snippet: Membrane was incubated overnight at 4°C with primary Abs against BDNF (1:100; Santa Cruz MATERNAL FRUCTOSE AND OFFSPRING BRAIN FUNCTION 2551 nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.201700783R R by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [23/10/2024].

Techniques: Expressing, Methylation, Gene Expression, DNA Methylation Assay

Figure 11. The putative mechanism for the adverse effects of maternal fructose. Early-life excessive fructose exposure induces impaired adult hippocampal neurogenesis and cognitive function in offspring. These phenomena may be a result of up-regulated DNA methylation in BDNF promoter regions.

Journal: The FASEB Journal

Article Title: Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter

doi: 10.1096/fj.201700783rr

Figure Lengend Snippet: Figure 11. The putative mechanism for the adverse effects of maternal fructose. Early-life excessive fructose exposure induces impaired adult hippocampal neurogenesis and cognitive function in offspring. These phenomena may be a result of up-regulated DNA methylation in BDNF promoter regions.

Article Snippet: Membrane was incubated overnight at 4°C with primary Abs against BDNF (1:100; Santa Cruz MATERNAL FRUCTOSE AND OFFSPRING BRAIN FUNCTION 2551 nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.201700783R R by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [23/10/2024].

Techniques: DNA Methylation Assay

Figure 10. Longitudinal changes in BDNF gene expression and DNA methylation between PD21 and -160. A) Decreased BDNF gene expression was observed at PD21 and -60, whereas no significant changes were observed at PD160 (n = 6–11/group). B) Excess maternal fructose intake induced hypermethylation of the BDNF promoter region at the CREB binding site between PD21 and -60 (n = 6–11/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.

Journal: The FASEB Journal

Article Title: Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter

doi: 10.1096/fj.201700783rr

Figure Lengend Snippet: Figure 10. Longitudinal changes in BDNF gene expression and DNA methylation between PD21 and -160. A) Decreased BDNF gene expression was observed at PD21 and -60, whereas no significant changes were observed at PD160 (n = 6–11/group). B) Excess maternal fructose intake induced hypermethylation of the BDNF promoter region at the CREB binding site between PD21 and -60 (n = 6–11/group). C, C group; F, F group. Values are presented as means 6 SD. *P , 0.05, **P , 0.01.

Article Snippet: Membrane was incubated overnight at 4°C with primary Abs against BDNF (1:100; Santa Cruz MATERNAL FRUCTOSE AND OFFSPRING BRAIN FUNCTION 2551 nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.201700783R R by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [23/10/2024].

Techniques: Gene Expression, DNA Methylation Assay, Binding Assay